| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 351 | Thermus thermophilus | GAGTTCCCCAGCTTGAGAGG | GTGAACCCATCGGTGACCA | 59.75 | 59.63 | 299 | 49 |
100.00%
|
100.00%
|
| Primer 352 | Thermus thermophilus | CGAAAACGGGATCGGCATG | AGGCTGTGGTAGACCTCCA | 59.65 | 59.53 | 198 | 49 |
100.00%
|
100.00%
|
| Primer 353 | Thermus thermophilus | CGAAAACGGGATCGGCAT | TGGGGTGGATGGGGATCTT | 58.19 | 59.91 | 173 | 49 |
100.00%
|
100.00%
|
| Primer 354 | Thermus thermophilus | AAAACGGGATCGGCATGG | TGGGGTGGATGGGGATCTT | 58.08 | 59.91 | 171 | 49 |
100.00%
|
100.00%
|
| Primer 355 | Thermus thermophilus | CATCGAGGGTCACGTCTACG | TGGGGTGGATGGGGATCTT | 59.97 | 59.91 | 203 | 49 |
100.00%
|
100.00%
|
| Primer 356 | Thermus thermophilus | GAAAACGGGATCGGCATGG | AGGCTGTGGTAGACCTCCA | 59.27 | 59.53 | 197 | 49 |
100.00%
|
100.00%
|
| Primer 357 | Thermus thermophilus | TGTTTAGGCTCGTGCGCT | ACCACGAGGAAGGGGAAGA | 59.66 | 59.84 | 208 | 49 |
100.00%
|
100.00%
|
| Primer 358 | Thermus thermophilus | TTACGAGCAACGCCTCCTG | TGTCCGTGGAGAAGACCCA | 60.08 | 60.15 | 212 | 49 |
100.00%
|
100.00%
|
| Primer 359 | Thermus thermophilus | TTACGAGCAACGCCTCCTG | ATCTCCAGGCACCACTCCA | 60.08 | 60.23 | 171 | 49 |
100.00%
|
100.00%
|
| Primer 360 | Thermus thermophilus | TCCTGAACCCCAACCTGGA | ACTTCACCACCATGACCCG | 60.07 | 59.63 | 252 | 49 |
100.00%
|
100.00%
|